Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 20883-1-AP
HT-29 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 20883-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 20883-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20883-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 20883-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 20883-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 20883-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 20883-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 20883-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 20883-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 20883-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 20883-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 20883-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 20883-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 20883-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 20883-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 20883-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 20883-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HT-29 using 20883-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPHB4 antibody (20883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

EPHB4 Polyclonal antibody

EPHB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20883-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MYK1, HTK, Ephrin type-B receptor 4, Ephrin type B receptor 4, EPH receptor B4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-29 cells, rat testis tissue, HeLa cells, HT-1080 cells, PC-3 cells, DU 145 cells
Positive IHC detected in	human lung cancer tissue, human pancreas tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HT-29 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 11 publications below
IF	See 8 publications below
FC	See 1 publications below

Product Information

20883-1-AP targets EPHB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10033 Product name: Recombinant human EPHB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 639-987 aa of BC052804 Sequence: KKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY 相同性解析による交差性が予測される生物種
Full Name	EPH receptor B4
Calculated molecular weight	987 aa, 108 kDa
Observed molecular weight	110-120 kDa
GenBank accession number	BC052804
Gene Symbol	EPHB4
Gene ID (NCBI)	2050
RRID	AB_10897158
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P54760
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
FC protocol for EPHB4 antibody 20883-1-AP	Download protocol
IF protocol for EPHB4 antibody 20883-1-AP	Download protocol
IHC protocol for EPHB4 antibody 20883-1-AP	Download protocol
WB protocol for EPHB4 antibody 20883-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Small Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres. Authors - Xiaomeng Guo View Article
human	IF	Drug Deliv Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging. Authors - Zuhua Wang View Article
	WB,IHC	J Cell Physiol Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling. Authors - Tiancheng Li View Article
human	WB	J Cell Mol Med Kinome multigenic panel identified novel druggable EPHB4-V871I somatic variant in high-risk neuroblastoma. Authors - Immacolata Andolfo View Article
human	FC	Phytomedicine Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1α pathways in breast cancer. Authors - Qi Su View Article
rat	IHC	Commun Biol The application of tissue-engineered fish swim bladder vascular graft. Authors - Hualong Bai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20883-1-AP

WB analysis of rat testis using 20883-1-AP

WB analysis of rat testis using 20883-1-AP

WB analysis of HeLa using 20883-1-AP

WB analysis of HT-1080 using 20883-1-AP

WB analysis of DU 145 using 20883-1-AP

WB analysis of DU 145 using 20883-1-AP

WB analysis of PC-3 using 20883-1-AP

WB analysis of PC-3 using 20883-1-AP

WB analysis of PC-3 using 20883-1-AP

IHC staining of human lung cancer using 20883-1-AP

IHC staining of human lung cancer using 20883-1-AP

IHC staining of human pancreas using 20883-1-AP

IHC staining of human pancreas using 20883-1-AP

IHC staining of human placenta using 20883-1-AP

IHC staining of human placenta using 20883-1-AP

IHC staining of human lung cancer using 20883-1-AP

IHC staining of human lung cancer using 20883-1-AP

IF Staining of HT-29 using 20883-1-AP

EPHB4 Polyclonal antibody

EPHB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres.

Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging.

Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling.

Kinome multigenic panel identified novel druggable EPHB4-V871I somatic variant in high-risk neuroblastoma.

Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1α pathways in breast cancer.

The application of tissue-engineered fish swim bladder vascular graft.