Validation Data Gallery
Tested Applications
| Positive WB detected in | L-929 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87904-1-RR targets EPHA2 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg8778 Product name: Recombinant mouse Epha2 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 26-538 aa of NM_010139.3 Sequence: KEVVLLDFAAMKGELGWLTHPYGKGWDLMQNIMDDMPIYMYSVCNVVSGDQDNWLRTNWVYREEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDVDYGTNFQKRQFTKIDTIAPDEITVSSDFEARNVKLNVEERMVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPEMLQSLARFPETIAVAVSDTQPLATVAGTCVDHAVVPYGGEGPLMHCTVDGEWLVPIGQCLCQEGYEKVEDACRACSPGFFKSEASESPCLECPEHTLPSTEGATSCQCEEGYFRAPEDPLSMSCTRPPSAPNYLTAIGMGAKVELRWTAPKDTGGRQDIVYSVTCEQCWPESGECGPCEASVRYSEPPHALTRTSVTVSDLEPHMNYTFAVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEDRSTTSLSVTWSIPVSQQSRVWKYEVTYRKKGDANSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSTEGSANMAV 相同性解析による交差性が予測される生物種 |
| Full Name | Eph receptor A2 |
| Calculated molecular weight | 109 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | NM_010139.3 |
| Gene Symbol | Epha2 |
| Gene ID (NCBI) | 13836 |
| RRID | AB_3745819 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q03145 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EPH receptor A2 (EPHA2) is a receptor tyrosine kinase (RTK) of the Eph receptor family that primarily binds glycosylphosphatidylinositol (GPI)-anchored ephrin-A ligands, especially ephrin-A1 (EFNA1), to mediate contact-dependent cell-cell signaling. In mice, Epha2 is broadly expressed in epithelial, endothelial, and selected stromal compartments, where it regulates cell adhesion, polarity, migration, proliferation, barrier organization, and tissue architecture.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EPHA2 antibody 87904-1-RR | Download protocol |
| WB protocol for EPHA2 antibody 87904-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |