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Validation Data Gallery

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  • Western Blot (WB) analysis of CHO cells using EPAC1 Polyclonal antibody (12572-1-AP)

    WB analysis of CHO using 12572-1-AP (same clone as 12572-1-PBS)

    CHO cells were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EPAC1 Polyclonal antibody (12572-1-AP)

    WB analysis using 12572-1-AP (same clone as 12572-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EPAC1 Polyclonal antibody (12572-1-AP)

    IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EPAC1 Polyclonal antibody (12572-1-AP)

    IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using EPAC1 Polyclonal antibody (12572-1-AP)

    IF Staining of mouse testis using 12572-1-AP (same clone as 12572-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using EPAC1 Polyclonal antibody (12572-1-AP)

    IF Staining of mouse testis using 12572-1-AP (same clone as 12572-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

EPAC1 Polyclonal antibody, PBS Only

EPAC1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 12572-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12572-1-PBS targets EPAC1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity human, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3240

Product name: Recombinant human RAPGEF3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 441-788 aa of BC017728

Sequence: HFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALE

相同性解析による交差性が予測される生物種
Full Name Rap guanine nucleotide exchange factor (GEF) 3
Calculated molecular weight 881 aa, 99 kDa
Observed molecular weight 99-104 kDa
GenBank accession numberBC017728
Gene Symbol EPAC1/RAPGEF3
Gene ID (NCBI) 10411
RRIDAB_11232407
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95398
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EPAC1 functions as a guanine nucleotide exchange factor (GEF), activating small GTPases like Rap1 and Rap2, leading to diverse cellular response. Epac proteins are cAMP sensors that regulate several pivotal cellular processes, including calcium handling, cell proliferation, cell survival, cell differentiation, cell polarization, cell adhesion events, gene transcription, secretion, ion transport, and neuronal signaling. It is a potential target for therapeutic intervention, such as metabolic disorders, cardiovascular diseases (PMID: 20007405, PMID: 20421979). EPAC1 also enhances brown fat growth and beige adipogenesis (PMID: 38195707).

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