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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293 cells using EP400 Polyclonal antibody (13858-1-AP)

    IP experiment of HEK-293 using 13858-1-AP (same clone as 13858-1-PBS)

    IP result of anti-EP400 (IP:13858-1-AP, 4ug; Detection:13858-1-AP 1:500) with HEK-293 cells lysate 1440 ug. This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using EP400 Polyclonal antibody (13858-1-AP)

    IHC staining of human colon using 13858-1-AP (same clone as 13858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13858-1-AP (EP400 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EP400 Polyclonal antibody (13858-1-AP)

    IF Staining of HeLa using 13858-1-AP (same clone as 13858-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EP400 antibody (13858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EP400 Polyclonal antibody (13858-1-AP)

    IF Staining of U2OS using 13858-1-AP (same clone as 13858-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EP400 antibody (13858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

EP400 Polyclonal antibody, PBS Only

EP400 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13858-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CAGH32, E1A-binding protein p400, EC:3.6.4.-, EP 400, hDomino

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13858-1-PBS targets EP400 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4832

Product name: Recombinant human EP400 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC037208

Sequence: MHHGTGPQNVQHQLQRSRACPGSEGEEQPAHPNPPPSPAAPFAPSASPSAPQSPSYQIQQLMNRSPATGQNVNITLQSVGPVVGGNQQITLAPLPLPSPTSPGFQFSAQPRRFEHGSPSYIQVTSPLSQQVQTQSPTQPSPGPGQALQNVRAGAPGPGLGLCSSSPTGGFVDASVLVRQISLSPSSGGHFVFQDGSGLTQIAQGAQVQLQHPGTPITVRERRPSQPHTQSGGTIHHLGPQSPAAAGGAGLQPLASPSHITTANLPPQISSIIQGQLVQQQQVLQGPPLPRPLGFERTPGV

相同性解析による交差性が予測される生物種
Full Name E1A binding protein p400
Calculated molecular weight 3160 aa, 344 kDa
Observed molecular weight344 kDa
GenBank accession numberBC037208
Gene Symbol EP400
Gene ID (NCBI) 57634
RRIDAB_3741847
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96L91
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

E1A binding protein p400 (EP400) is a SWR1-class ATP-dependent chromatin remodeling protein originally identified as an interacting partner for the adenovirus E1A protein required for viral replication and cellular transformation. EP400 plays a crucial role in promoting double-strand break (DSB) repair through homologous recombination (PMID: 26669263, 37922899). Depletion of Ep400 in oocytes led to accumulated DSBs, causing abnormal aggregation and dysfunction of cytoplasmic organelles, resulting in impaired oocyte development and declined oocyte quality (PMID: 38493496).

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