Validation Data Gallery
Tested Applications
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, U2OS cells |
Recommended dilution
Application | Dilution |
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Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
13858-1-AP targets EP400 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EP400 fusion protein Ag4832 相同性解析による交差性が予測される生物種 |
Full Name | E1A binding protein p400 |
Calculated molecular weight | 3160 aa, 344 kDa |
Observed molecular weight | 344 kDa |
GenBank accession number | BC037208 |
Gene Symbol | EP400 |
Gene ID (NCBI) | 57634 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96L91 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
E1A binding protein p400 (EP400) is a SWR1-class ATP-dependent chromatin remodeling protein originally identified as an interacting partner for the adenovirus E1A protein required for viral replication and cellular transformation. EP400 plays a crucial role in promoting double-strand break (DSB) repair through homologous recombination (PMID: 26669263, 37922899). Depletion of Ep400 in oocytes led to accumulated DSBs, causing abnormal aggregation and dysfunction of cytoplasmic organelles, resulting in impaired oocyte development and declined oocyte quality (PMID: 38493496).
Protocols
Product Specific Protocols | |
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IHC protocol for EP400 antibody 13858-1-AP | Download protocol |
IF protocol for EP400 antibody 13858-1-AP | Download protocol |
IP protocol for EP400 antibody 13858-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |