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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293 cells using EP400 Polyclonal antibody (13858-1-AP)

    IP experiment of HEK-293 using 13858-1-AP

    IP result of anti-EP400 (IP:13858-1-AP, 4ug; Detection:13858-1-AP 1:500) with HEK-293 cells lysate 1440 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using EP400 Polyclonal antibody (13858-1-AP)

    IHC staining of human colon using 13858-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13858-1-AP (EP400 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EP400 Polyclonal antibody (13858-1-AP)

    IF Staining of HeLa using 13858-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EP400 antibody (13858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EP400 Polyclonal antibody (13858-1-AP)

    IF Staining of U2OS using 13858-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EP400 antibody (13858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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EP400 Polyclonal antibody

EP400 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 13858-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, ELISA

Synonyms

CAGH32, E1A-binding protein p400, EC:3.6.4.-, EP 400, hDomino

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13858-1-AP targets EP400 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EP400 fusion protein Ag4832 相同性解析による交差性が予測される生物種
Full Name E1A binding protein p400
Calculated molecular weight 3160 aa, 344 kDa
Observed molecular weight344 kDa
GenBank accession numberBC037208
Gene Symbol EP400
Gene ID (NCBI) 57634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96L91
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

E1A binding protein p400 (EP400) is a SWR1-class ATP-dependent chromatin remodeling protein originally identified as an interacting partner for the adenovirus E1A protein required for viral replication and cellular transformation. EP400 plays a crucial role in promoting double-strand break (DSB) repair through homologous recombination (PMID: 26669263, 37922899). Depletion of Ep400 in oocytes led to accumulated DSBs, causing abnormal aggregation and dysfunction of cytoplasmic organelles, resulting in impaired oocyte development and declined oocyte quality (PMID: 38493496).

Protocols

Product Specific Protocols
IHC protocol for EP400 antibody 13858-1-APDownload protocol
IF protocol for EP400 antibody 13858-1-APDownload protocol
IP protocol for EP400 antibody 13858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols