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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse spleen tissue using ENGASE Polyclonal antibody (31784-1-AP)

    IHC staining of mouse spleen using 31784-1-AP (same clone as 31784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using ENGASE Polyclonal antibody (31784-1-AP)

    IHC staining of mouse spleen using 31784-1-AP (same clone as 31784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using ENGASE Polyclonal antibody (31784-1-AP)

    IHC staining of mouse thymus using 31784-1-AP (same clone as 31784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using ENGASE Polyclonal antibody (31784-1-AP)

    IHC staining of mouse thymus using 31784-1-AP (same clone as 31784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using ENGASE Polyclonal antibody (31784-1-AP)

    IF Staining of U-251 using 31784-1-AP (same clone as 31784-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using ENGASE antibody (31784-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

ENGASE Polyclonal antibody, PBS Only

ENGASE Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 31784-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

EC:3.2.1.96, Cytosolic endo-beta-N-acetylglucosaminidase

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31784-1-PBS targets ENGASE in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34633

Product name: Recombinant human ENGASE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-200 aa of NM_001042573

Sequence: IKDEEEETVFREVVSFSPDPLPVRYYDKDTTKPISFYLSSLEELLAWKPRLEDGFNVALEPLACRQPPLSSQRPRTLLCHDMMGGYLDDRFIQGSVVQTPYAFYHWQCIDVFVYFSHHTVTIPPVGWTNTAHRHGVCVLGTFITEWNEGGR

相同性解析による交差性が予測される生物種
Full Name endo-beta-N-acetylglucosaminidase
Calculated molecular weight84 kDa
GenBank accession numberNM_001042573
Gene Symbol ENGASE
Gene ID (NCBI) 64772
RRIDAB_3670113
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8NFI3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Endo-β-N-acetylglucosaminidase (ENGASE) is an enzyme that belongs to glycoside hydrolase (GH) families 18 and 85, and is widely distributed in bacteria, fungi, and higher-order species. ENGASE hydrolyzes the β1-4 linkage of the N, N-diacetylchitobiose (GlcNAcβ1-4GlcNAc) region of the N-linked glycans present on glycoproteins (PMID: 36435009). ENGASE is widely expressed, with higher expression in thymus and spleen (Refer to Uniprot).

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