Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse spleen tissue, mouse thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31784-1-AP targets ENGASE in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34633 Product name: Recombinant human ENGASE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-200 aa of NM_001042573 Sequence: IKDEEEETVFREVVSFSPDPLPVRYYDKDTTKPISFYLSSLEELLAWKPRLEDGFNVALEPLACRQPPLSSQRPRTLLCHDMMGGYLDDRFIQGSVVQTPYAFYHWQCIDVFVYFSHHTVTIPPVGWTNTAHRHGVCVLGTFITEWNEGGR 相同性解析による交差性が予測される生物種 |
| Full Name | endo-beta-N-acetylglucosaminidase |
| Calculated molecular weight | 84 kDa |
| GenBank accession number | NM_001042573 |
| Gene Symbol | ENGASE |
| Gene ID (NCBI) | 64772 |
| RRID | AB_3670113 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8NFI3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Endo-β-N-acetylglucosaminidase (ENGASE) is an enzyme that belongs to glycoside hydrolase (GH) families 18 and 85, and is widely distributed in bacteria, fungi, and higher-order species. ENGASE hydrolyzes the β1-4 linkage of the N, N-diacetylchitobiose (GlcNAcβ1-4GlcNAc) region of the N-linked glycans present on glycoproteins (PMID: 36435009). ENGASE is widely expressed, with higher expression in thymus and spleen (Refer to Uniprot).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ENGASE antibody 31784-1-AP | Download protocol |
| IHC protocol for ENGASE antibody 31784-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |