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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using ENC1 Polyclonal antibody (15007-1-AP)

    WB analysis of SH-SY5Y using 15007-1-AP (same clone as 15007-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15007-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using ENC1 Polyclonal antibody (15007-1-AP)

    WB analysis of Neuro-2a using 15007-1-AP (same clone as 15007-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15007-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ENC1 Polyclonal antibody (15007-1-AP)

    WB analysis of MCF-7 using 15007-1-AP (same clone as 15007-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15007-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ENC1 Polyclonal antibody (15007-1-AP)

    WB analysis of mouse brain using 15007-1-AP (same clone as 15007-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15007-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using ENC1 Polyclonal antibody (15007-1-AP)

    WB analysis of human lung using 15007-1-AP (same clone as 15007-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15007-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ENC1 Polyclonal antibody (15007-1-AP)

    IP experiment of mouse brain using 15007-1-AP (same clone as 15007-1-PBS)

    IP result of anti-ENC1 (IP:15007-1-AP, 4ug; Detection:15007-1-AP 1:1000) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 15007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ENC1 Polyclonal antibody (15007-1-AP)

    IHC staining of human pancreas using 15007-1-AP (same clone as 15007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ENC1 Polyclonal antibody (15007-1-AP)

    IHC staining of human pancreas using 15007-1-AP (same clone as 15007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15007-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ENC1 Polyclonal antibody, PBS Only

ENC1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15007-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15007-1-PBS targets ENC1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6607

Product name: Recombinant human ENC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 240-589 aa of BC000418

Sequence: LLPAIYLMENVAMEELITKQRKSKEIVEEAIRCKLKILQNDGVVTSLCARPRKTGHALFLLGGQTFMCDKLYLVDQKAKEIIPKADIPSPRKEFSACAIGCKVYITGGRGSENGVSKDVWVYDTLHEEWSKAAPMLVARFGHGSAELKHCLYVVGGHTAATGCLPASPSVSLKQVEHYDPTINKWTMVAPLREGVSNAAVVSAKLKLFAFGGTSVSHDKLPKVQCYDQCENRWTVPATCPQPWRYTAAAVLGNQIFIMGGDTEFSACSAYKFNSETYQWTKVGDVTAKRMSCHAVASGNKLYVVGGYFGIQRCKTLDCYDPTLDVWNSITTVPYSLIPTAFVSTWKHLPS

相同性解析による交差性が予測される生物種
Full Name ectodermal-neural cortex (with BTB-like domain)
Calculated molecular weight 66 kDa
Observed molecular weight 67 kDa, 57 kDa
GenBank accession numberBC000418
Gene Symbol ENC1
Gene ID (NCBI) 8507
RRIDAB_2098751
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14682
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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