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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

ENC1 Polyclonal antibody

ENC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15007-1-AP

Synonyms

CCL28, ENC 1, ENC1, Kelch like protein 37, KLHL35, KLHL37, NRPB, Nuclear matrix protein NRP/B, p53 induced gene 10 protein, PIG10, TP53I10

Validation Data Gallery

Applications:
  • WB analysis of SH-SY5Y using 15007-1-AP

    WB analysis of SH-SY5Y using 15007-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Neuro-2a using 15007-1-AP

    WB analysis of Neuro-2a using 15007-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 15007-1-AP

    WB analysis of MCF-7 using 15007-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 15007-1-AP

    WB analysis of mouse brain using 15007-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of human lung using 15007-1-AP

    WB analysis of human lung using 15007-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 15007-1-AP

    IP experiment of mouse brain using 15007-1-AP

    IP result of anti-ENC1 (IP:15007-1-AP, 4ug; Detection:15007-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

  • IHC staining of human pancreas using 15007-1-AP

    IHC staining of human pancreas using 15007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ENC1 antibody included
  • IHC staining of human pancreas using 15007-1-AP

    IHC staining of human pancreas using 15007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ENC1 antibody included

Tested Applications

Positive WB detected inSH-SY5Y cells, Neuro-2a cells, MCF-7 cells, mouse brain tissue, human lung tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ENC1 Ready-To-Use IHC Kit. ENC1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15007-1-AP targets ENC1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENC1 fusion protein Ag6607 相同性解析による交差性が予測される生物種
Full Name ectodermal-neural cortex (with BTB-like domain)
Calculated molecular weight 66 kDa
Observed molecular weight 67 kDa, 57 kDa
GenBank accession numberBC000418
Gene symbol ENC1
Gene ID (NCBI) 8507
RRIDAB_2098751
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ENC1 antibody 15007-1-APDownload protocol
IHC protocol for ENC1 antibody 15007-1-APDownload protocol
IP protocol for ENC1 antibody 15007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Int J Mol Sci

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

Authors - Marianna Naki
View Article
humanWB, IHC

Front Cell Dev Biol

ENC1 Facilitates Colorectal Carcinoma Tumorigenesis and Metastasis via JAK2/STAT5/AKT Axis-Mediated Epithelial Mesenchymal Transition and Stemness.

Authors - Ying Cui
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Hee (Verified Customer) (08-16-2019)

Very good

  • Applications: Western Blot,