Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis using 67095-1-Ig (same clone as 67095-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 67095-1-Ig (same clone as 67095-1-PBS)
IP result of anti-EIF3E (IP:67095-1-Ig, 5ug; Detection:67095-1-Ig 1:2000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67095-1-Ig (same clone as 67095-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3E antibody (67095-1-Ig, Clone: 1C6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

EIF3E Monoclonal antibody, PBS Only

EIF3E Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 67095-1-PBS

Clone No.1C6C8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

1C6C8, eIF-3 p48, EIF3S6, INT6, Viral integration site protein INT-6 homolog

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

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MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67095-1-PBS targets EIF3E in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28523 Product name: Recombinant human EIF3E protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 146-445 aa of BC000734 Sequence: GAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 3, subunit E
Calculated molecular weight	48 kDa
Observed molecular weight	48-52 kDa
GenBank accession number	BC000734
Gene Symbol	EIF3E
Gene ID (NCBI)	3646
RRID	AB_2882400
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P60228
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

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