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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF3E Monoclonal antibody

EIF3E Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1C6C8

Cat no : 67095-1-Ig

Synonyms

eIF 3 p48, eIF3 p46, EIF3 P48, EIF3E, EIF3S6, INT6

Validation Data Gallery

Applications:
  • WB analysis using 67095-1-Ig

    WB analysis using 67095-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of HeLa using 67095-1-Ig

    IP experiment of HeLa using 67095-1-Ig

    IP result of anti-EIF3E (IP:67095-1-Ig, 5ug; Detection:67095-1-Ig 1:2000) with HeLa cells lysate 1600 ug.

Tested Applications

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EIF3E Ready-To-Use IHC Kit. EIF3E primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

67095-1-Ig targets EIF3E in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF3E fusion protein Ag28523 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit E
Calculated molecular weight 48 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC000734
Gene symbol EIF3E
Gene ID (NCBI) 3646
RRIDAB_2882400
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF3E antibody 67095-1-IgDownload protocol
IP protocol for EIF3E antibody 67095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols