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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2B1 Monoclonal antibody (68824-1-Ig)

    WB analysis using 68824-1-Ig (same clone as 68824-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68824-1-Ig (EIF2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68824-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using EIF2B1 Monoclonal antibody (68824-1-Ig)

    WB analysis of NIH/3T3 using 68824-1-Ig (same clone as 68824-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68824-1-Ig (EIF2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68824-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2B1 Monoclonal antibody (68824-1-Ig)

    IF Staining of HeLa using 68824-1-Ig (same clone as 68824-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2B1 antibody (68824-1-Ig, Clone: 1H6A3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68824-1-PBS in a different storage buffer formulation.

EIF2B1 Monoclonal antibody, PBS Only

EIF2B1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68824-1-PBS
Clone No.1H6A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF/ICC, ELISA

Synonyms

EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha, EIF 2B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68824-1-PBS targets EIF2B1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12493

Product name: Recombinant human EIF2B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-305 aa of BC103763

Sequence: MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDELIKLYL

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
Calculated molecular weight 305 aa, 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC103763
Gene Symbol EIF2B1
Gene ID (NCBI) 1967
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ14232
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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