Validation Data Gallery
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Tested Applications
| Positive WB detected in | NIH/3T3 cells, U2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68824-1-Ig targets EIF2B1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12493 Product name: Recombinant human EIF2B1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-305 aa of BC103763 Sequence: MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDELIKLYL 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa |
| Calculated molecular weight | 305 aa, 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC103763 |
| Gene Symbol | EIF2B1 |
| Gene ID (NCBI) | 1967 |
| RRID | AB_3670435 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14232 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF2B1 antibody 68824-1-Ig | Download protocol |
| WB protocol for EIF2B1 antibody 68824-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |