CoraLite® Plus 488-conjugated EIF2AK1 Monoclonal antibody
EIF2AK1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2H1F3
Cat no : CL488-67674
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-67674 targets EIF2AK1 in IF applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EIF2AK1 fusion protein Ag16970 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 2-alpha kinase 1 |
| Calculated molecular weight | 630 aa, 71 kDa |
| Observed molecular weight | 71 kDa |
| GenBank accession number | BC006524 |
| Gene symbol | EIF2AK1 |
| Gene ID (NCBI) | 27102 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 EIF2AK1 antibody CL488-67674 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
