Validation Data Gallery
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
Product Information
EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. It has been shown that EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.
| Source | CHO cells-derived Human EGFR protein (25-645 aa) (Accession #P00533) with a mutated Human IgG1 FcZero backbone |
| Isotype | Recombinant Human IgG1 |
| Tested Reactivity | Human |
| Applications | FC |
| Target Cell | EGFR-specific CAR-T cells, EGFR-specific CAR-NK cells |
| Backbone | Human IgG1 FcZero backbone |
| Site Specific Conjugation | Site specific conjugated with Biotin |
| Form | Liquid |
| GeneID | 1956 |
| UNIPROT ID | P00533 |
| Conjugate | Biotin |
| Excitation/Emission Maxima Wavelengths | - |
| Storage Buffer | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |