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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis of mouse brain using 24729-1-AP (same clone as 24729-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24729-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis using 24729-1-AP (same clone as 24729-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24729-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis of fetal human brain using 24729-1-AP (same clone as 24729-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24729-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    IHC staining of human kidney using 24729-1-AP (same clone as 24729-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24729-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    IHC staining of human kidney using 24729-1-AP (same clone as 24729-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24729-1-PBS in a different storage buffer formulation.

EFTUD1 Polyclonal antibody, PBS Only

EFTUD1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24729-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EFL1, Elongation factor Tu GTP-binding domain-containing protein 1, Elongation factor-like 1, Elongation factor-like GTPase 1, FAM42A

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24729-1-PBS targets EFTUD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20371

Product name: Recombinant human EFTUD1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 772-1120 aa of BC156743

Sequence: LIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

相同性解析による交差性が予測される生物種
Full Name elongation factor Tu GTP binding domain containing 1
Calculated molecular weight 1120 aa, 125 kDa
Observed molecular weight 125-135 kDa
GenBank accession numberBC156743
Gene Symbol EFTUD1
Gene ID (NCBI) 79631
RRIDAB_2879693
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z2Z2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EFTUD1 (Elongation Factor Tu GTP Binding Domain Containing Protein 1) is a protein involved in ribosome biogenesis and translation activation. It is a member of the GTP-binding elongation factor family. EFTUD1 plays a crucial role in the release of eukaryotic translation initiation factor 6 (eIF6) from 60S ribosome subunits, allowing these subunits to join with 40S subunits to form actively translating 80S complexes. (PMID: 25015090)

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