×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis of mouse brain using 24729-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis using 24729-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis of fetal human brain using 24729-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    IHC staining of human kidney using 24729-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    IHC staining of human kidney using 24729-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 40x lens).

EFTUD1 Polyclonal antibody

EFTUD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24729-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EFL1, Elongation factor Tu GTP-binding domain-containing protein 1, Elongation factor-like 1, Elongation factor-like GTPase 1, FAM42A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24729-1-AP targets EFTUD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20371

Product name: Recombinant human EFTUD1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 772-1120 aa of BC156743

Sequence: LIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

相同性解析による交差性が予測される生物種
Full Name elongation factor Tu GTP binding domain containing 1
Calculated molecular weight 1120 aa, 125 kDa
Observed molecular weight 125-135 kDa
GenBank accession numberBC156743
Gene Symbol EFTUD1
Gene ID (NCBI) 79631
RRIDAB_2879693
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z2Z2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EFTUD1 (Elongation Factor Tu GTP Binding Domain Containing Protein 1) is a protein involved in ribosome biogenesis and translation activation. It is a member of the GTP-binding elongation factor family. EFTUD1 plays a crucial role in the release of eukaryotic translation initiation factor 6 (eIF6) from 60S ribosome subunits, allowing these subunits to join with 40S subunits to form actively translating 80S complexes. (PMID: 25015090)

Protocols

Product Specific Protocols
IHC protocol for EFTUD1 antibody 24729-1-APDownload protocol
WB protocol for EFTUD1 antibody 24729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med

Functional impact of the eIF6 N106S mutation on ribosome biogenesis in wild type and Shwachman-Diamond syndrome cells.

Authors - Alexis Bertrand
View Article
||
New chat

Able™

正在加载，请稍候...