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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated EEF1G Monoclonal ant (CL488-68148)

    IF Staining of HeLa using CL488-68148

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 EEF1G antibody (CL488-68148, Clone: 1G7C6 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated EEF1G Monoclonal antibody

EEF1G Monoclonal Antibody for IF/ICC
Cat No. CL488-68148
Clone No.1G7C6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rabbit

Applications

IF/ICC

Synonyms

eEF 1B gamma, EEF1G, EF 1 gamma, EF1G, Elongation factor 1 gamma, GIG35

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68148 targets EEF1G in IF/ICC applications and shows reactivity with Human, Pig, Rabbit samples.

Tested Reactivity Human, Pig, Rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0218

Product name: Recombinant human EEF1G protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-400 aa of BC006520

Sequence: GLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWR

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 gamma
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC006520
Gene Symbol EEF1G
Gene ID (NCBI) 1937
RRIDAB_3084419
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP26641
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EEF1G antibody CL488-68148Download protocol
Standard Protocols
Click here to view our Standard Protocols