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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated EEF1D Monoclonal ant (CL488-60085)

    IF Staining of HepG2 using CL488-60085

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-60085 (EEF1D antibody), at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated EEF1D Monoclonal ant (CL488-60085)

    IF Staining of HeLa using CL488-60085

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-60085 (EEF1D antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated EEF1D Monoclonal ant (CL488-60085)

    FC experiment of MCF-7 using CL488-60085

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EEF1D (CL488-60085, Clone:3B1B11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated EEF1D Monoclonal ant (CL488-60085)

    FC experiment of HeLa using CL488-60085

    1X10^6 HeLa cells were intracellularly stained with 1 ug CoraLite® Plus 488 Anti-Human EEF1D (CL488-60085, Clone:3B1B11) (red), or 1 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated EEF1D Monoclonal antibody

EEF1D Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-60085
Clone No.3B1B11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

EF-1-delta, EF1D, EF 1D, EF 1 delta, Antigen NY-CO-4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60085 targets EEF1D in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0983

Product name: Recombinant human EEF1D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 347-647 aa of BC007847

Sequence: SHRPGPRSGLSVSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Calculated molecular weight 31 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC007847
Gene Symbol EEF1D
Gene ID (NCBI) 1936
RRIDAB_2883100
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP29692
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EEF1D antibody CL488-60085Download protocol
Standard Protocols
Click here to view our Standard Protocols