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Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using EDEM3 Polyclonal antibody (27310-1-AP)

    WB analysis of 3T3-L1 using 27310-1-AP (same clone as 27310-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27310-1-AP (EDEM3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27310-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using EDEM3 Polyclonal antibody (27310-1-AP)

    IP experiment of HEK-293 using 27310-1-AP (same clone as 27310-1-PBS)

    IP result of anti-EDEM3 (IP:27310-1-AP, 4ug; Detection:27310-1-AP 1:300) with HEK-293 cells lysate 2600ug. This data was developed using the same antibody clone with 27310-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EDEM3 Polyclonal antibody (27310-1-AP)

    IHC staining of human stomach cancer using 27310-1-AP (same clone as 27310-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27310-1-AP (EDEM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27310-1-PBS in a different storage buffer formulation.

EDEM3 Polyclonal antibody, PBS Only

EDEM3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27310-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ER degradation-enhancing alpha-mannosidase-like protein 3, EC:3.2.1.113, C1orf22, Alpha-1,2-mannosidase EDEM3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27310-1-PBS targets EDEM3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26123

Product name: Recombinant human EDEM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-80 aa of BC016464

Sequence: DNAASISPSEQTSNPTENHETTNLNGECTDLDNQLQEQSETEEDSNPNVSWGKKVQPIDSILADWNEDIEAFEMMEKDEL

相同性解析による交差性が予測される生物種
Full Name ER degradation enhancer, mannosidase alpha-like 3
Calculated molecular weight 105 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC016464
Gene Symbol EDEM3
Gene ID (NCBI) 80267
RRIDAB_2880839
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZQ6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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