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Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using EDEM3 Polyclonal antibody (27310-1-AP)

    WB analysis of 3T3-L1 using 27310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27310-1-AP (EDEM3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using EDEM3 Polyclonal antibody (27310-1-AP)

    IP experiment of HEK-293 using 27310-1-AP

    IP result of anti-EDEM3 (IP:27310-1-AP, 4ug; Detection:27310-1-AP 1:300) with HEK-293 cells lysate 2600ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EDEM3 Polyclonal antibody (27310-1-AP)

    IHC staining of human stomach cancer using 27310-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27310-1-AP (EDEM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EDEM3 Polyclonal antibody

EDEM3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27310-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

ER degradation-enhancing alpha-mannosidase-like protein 3, EC:3.2.1.113, C1orf22, Alpha-1,2-mannosidase EDEM3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in3T3-L1 cells, HEK-293 cell
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27310-1-AP targets EDEM3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26123

Product name: Recombinant human EDEM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-80 aa of BC016464

Sequence: DNAASISPSEQTSNPTENHETTNLNGECTDLDNQLQEQSETEEDSNPNVSWGKKVQPIDSILADWNEDIEAFEMMEKDEL

相同性解析による交差性が予測される生物種
Full Name ER degradation enhancer, mannosidase alpha-like 3
Calculated molecular weight 105 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC016464
Gene Symbol EDEM3
Gene ID (NCBI) 80267
RRIDAB_2880839
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZQ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EDEM3 antibody 27310-1-APDownload protocol
IHC protocol for EDEM3 antibody 27310-1-APDownload protocol
IP protocol for EDEM3 antibody 27310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols