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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EB3 Polyclonal antibody (23974-1-AP)

    WB analysis using 23974-1-AP (same clone as 23974-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23974-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using EB3 Polyclonal antibody (23974-1-AP)

    IHC staining of human skeletal muscle using 23974-1-AP (same clone as 23974-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23974-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EB3 Polyclonal antibody (23974-1-AP)

    IHC staining of mouse brain using 23974-1-AP (same clone as 23974-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23974-1-AP (EB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23974-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EB3 Polyclonal antibody (23974-1-AP)

    IHC staining of mouse brain using 23974-1-AP (same clone as 23974-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23974-1-AP (EB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23974-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using EB3 Polyclonal antibody (23974-1-AP)

    IHC staining of human skeletal muscle using 23974-1-AP (same clone as 23974-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23974-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using EB3 Polyclonal antibody (23974-1-AP)

    IF Staining of mouse testis using 23974-1-AP (same clone as 23974-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EB3 antibody (23974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23974-1-PBS in a different storage buffer formulation.

EB3 Polyclonal antibody, PBS Only

EB3 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 23974-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

End binding protein 3, End-binding protein 3, MAPRE3, Microtubule-associated protein RP/EB family member 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23974-1-PBS targets EB3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21148

Product name: Recombinant human MAPRE3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-281 aa of BC011557

Sequence: MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYCQFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDNFEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSPTGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHETDAQILELNQQLVDLKLTVDGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY

相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein, RP/EB family, member 3
Calculated molecular weight 32 kDa
Observed molecular weight 30-36 kDa
GenBank accession numberBC011557
Gene Symbol EB3
Gene ID (NCBI) 22924
RRIDAB_2879385
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9UPY8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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