Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of mouse brain using 23974-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 23974-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 23974-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 23974-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).
IHC staining of mouse brain using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23974-1-AP (EB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 23974-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EB3 antibody (23974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

EB3 Polyclonal antibody

EB3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 23974-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

EB3, End binding protein 3, MAPRE3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IHC detected in	human skeletal muscle tissue, human brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 1 publications below

Product Information

23974-1-AP targets EB3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21148 Product name: Recombinant human MAPRE3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-281 aa of BC011557 Sequence: MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYCQFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDNFEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSPTGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHETDAQILELNQQLVDLKLTVDGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY 相同性解析による交差性が予測される生物種
Full Name	microtubule-associated protein, RP/EB family, member 3
Calculated molecular weight	32 kDa
Observed molecular weight	30-36 kDa
GenBank accession number	BC011557
Gene Symbol	EB3
Gene ID (NCBI)	22924
RRID	AB_2879385
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q9UPY8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for EB3 antibody 23974-1-AP	Download protocol
IHC protocol for EB3 antibody 23974-1-AP	Download protocol
WB protocol for EB3 antibody 23974-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. Authors - Hai-Qing Tu View Article
mouse	WB	Cell Tissue Res Filamin-A-interacting protein 1 (FILIP1) is a dual compartment protein linking myofibrils and microtubules during myogenic differentiation and upon mechanical stress Authors - Valentina Grande View Article
rat	IF	Neural Regen Res The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons Authors - Shi-Yan Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 23974-1-AP

WB analysis of mouse brain using 23974-1-AP

WB analysis of mouse brain using 23974-1-AP

WB analysis of rat brain using 23974-1-AP

IHC staining of human skeletal muscle using 23974-1-AP

IHC staining of human skeletal muscle using 23974-1-AP

IHC staining of human brain using 23974-1-AP

IHC staining of human brain using 23974-1-AP

IHC staining of mouse brain using 23974-1-AP

IF Staining of mouse testis using 23974-1-AP

EB3 Polyclonal antibody

EB3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Filamin-A-interacting protein 1 (FILIP1) is a dual compartment protein linking myofibrils and microtubules during myogenic differentiation and upon mechanical stress

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons