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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EAF2 Polyclonal antibody (11172-1-AP)

    WB analysis of HEK-293 using 11172-1-AP (same clone as 11172-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11172-1-AP (EAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using EAF2 Polyclonal antibody (11172-1-AP)

    WB analysis of mouse kidney using 11172-1-AP (same clone as 11172-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11172-1-AP (EAF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EAF2 Polyclonal antibody (11172-1-AP)

    IHC staining of human breast cancer using 11172-1-AP (same clone as 11172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EAF2 Polyclonal antibody (11172-1-AP)

    IHC staining of human breast cancer using 11172-1-AP (same clone as 11172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EAF2 Polyclonal antibody (11172-1-AP)

    IF Staining of HepG2 using 11172-1-AP (same clone as 11172-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 11172-1-AP (EAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

EAF2 Polyclonal antibody, PBS Only

EAF2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11172-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11172-1-PBS targets EAF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1637

Product name: Recombinant human EAF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC014209

Sequence: MNSAAGFSHLDRRERVLKLGESFEKQPRCAFHTVRYDFKPASIDTSSEGYLEVGEGEQVTITLPNIEGSTPPVTVFKGSKKPYLKECILIINHDTGECRLEKLSSNITVKKTRVEGSSKIQYRKEQQQQQMWNSARTPNLVKHSPSEDKMSPASPIDDIERELKAEASLMDQMSSCDSSSDSKSSSSSSSEDSSSDSEDEDCKSSTSDTGNCVSGHPTMTQYRIPDIDASHNRFRDNSGLLMNTLRNDLQLSESGSDSDD

相同性解析による交差性が予測される生物種
Full Name ELL associated factor 2
Calculated molecular weight 42 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC014209
Gene Symbol EAF2
Gene ID (NCBI) 55840
RRIDAB_2097560
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CJ1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330). Catalog#11172-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF2.

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