• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EAF2 Polyclonal antibody

EAF2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11172-1-AP

Synonyms

BM040, EAF2, ELL associated factor 2, TRAITS, U19

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 11172-1-AP

    WB analysis of HEK-293 using 11172-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11172-1-AP (EAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 11172-1-AP

    WB analysis of mouse kidney using 11172-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11172-1-AP (EAF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11172-1-AP

    IHC staining of human breast cancer using 11172-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11172-1-AP

    IHC staining of human breast cancer using 11172-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11172-1-AP

    IF Staining of HepG2 using 11172-1-AP

    Immunofluorescent analysis of HepG2 cells using 11172-1-AP (EAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11172-1-AP targets EAF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EAF2 fusion protein Ag1637 相同性解析による交差性が予測される生物種
Full Name ELL associated factor 2
Calculated molecular weight 42 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC014209
Gene symbol EAF2
Gene ID (NCBI) 55840
RRIDAB_2097560
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis.The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330).Catalog#11172-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF2.

Protocols

Product Specific Protocols
WB protocol for EAF2 antibody 11172-1-APDownload protocol
IHC protocol for EAF2 antibody 11172-1-APDownload protocol
IF protocol for EAF2 antibody 11172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Cell

Inherited blood cancer predisposition through altered transcription elongation

Authors - Jiawei Zhao
View Article