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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    WB analysis of mouse ovary using 12686-1-AP (same clone as 12686-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    WB analysis of mouse ovary using 12686-1-AP (same clone as 12686-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    WB analysis of human brain using 12686-1-AP (same clone as 12686-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    WB analysis of mouse testis using 12686-1-AP (same clone as 12686-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    IHC staining of mouse brain using 12686-1-AP (same clone as 12686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    IHC staining of mouse brain using 12686-1-AP (same clone as 12686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    IHC staining of human testis using 12686-1-AP (same clone as 12686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    IHC staining of human testis using 12686-1-AP (same clone as 12686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    IHC staining of human brain using 12686-1-AP (same clone as 12686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using EAAT3 Polyclonal antibody (12686-1-AP)

    IHC staining of human brain using 12686-1-AP (same clone as 12686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EAAT3 Polyclonal antibody, PBS Only

EAAT3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12686-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

SLC1A1, EAAT 3, Excitatory amino-acid carrier 1, HEAAC1, Slc1 a1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12686-1-PBS targets EAAT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3379

Product name: Recombinant human SLC1A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 430-524 aa of BC033040

Sequence: AEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF

相同性解析による交差性が予測される生物種
Full Name solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Calculated molecular weight 524 aa, 57 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC033040
Gene Symbol EAAT3
Gene ID (NCBI) 6505
RRIDAB_2188001
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43005
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EAAT3 (Excitatory Amino Acid Transporter 3), encoded by human SLC1A1 (Gene ID: 6505), is a neuronal high-affinity glutamate transporter of the SLC1 family. It is expressed mainly in CNS neurons, and also in intestinal and renal epithelial cells. EAAT3 is the only EAAT subtype that efficiently transports L-cysteine, supplying substrate for neuronal glutathione (GSH) synthesis and antioxidant defense. Its transport cycle couples uptake of L-glutamate, L-aspartate, or L-cysteine to 3 Na⁺/1 H⁺ co-transport and 1 K⁺ counter-transport. EAAT3 also contributes to metabolic reprogramming in activated B cells and cancer cells, and can transport the oncometabolite R-2-hydroxyglutarate (R-2HG). SLC1A1 mutations are linked to dicarboxylic aminoaciduria and schizophrenia susceptibility.

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