Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse ovary using 12686-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 12686-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12686-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12686-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 12686-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12686-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 12686-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12686-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 12686-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 12686-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

EAAT3 Polyclonal antibody

EAAT3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12686-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Synonyms

SLC1A1, EAAT 3, Excitatory amino-acid carrier 1, HEAAC1, Slc1 a1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse ovary tissue, human brain tissue, mouse testis tissue
Positive IHC detected in	mouse brain tissue, human brain tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

12686-1-AP targets EAAT3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig, chicken, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3379 Product name: Recombinant human SLC1A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 430-524 aa of BC033040 Sequence: AEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Calculated molecular weight	524 aa, 57 kDa
Observed molecular weight	66 kDa
GenBank accession number	BC033040
Gene Symbol	EAAT3
Gene ID (NCBI)	6505
RRID	AB_2188001
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P43005
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for EAAT3 antibody 12686-1-AP	Download protocol
WB protocol for EAAT3 antibody 12686-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Cancer Res Dysregulated Glutamate Transporter SLC1A1 Propels Cystine Uptake via Xc- for Glutathione Synthesis in Lung Cancer. Authors - Wenzheng Guo View Article
mouse	WB	Life Sci Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction Authors - Yu Ma View Article
mouse	WB	Neuropharmacology Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease. Authors - Yun-Long Zhang View Article
mouse	WB	J Alzheimers Dis S-Adenosylmethionine Alleviates Amyloid-β-Induced Neural Injury by Enhancing Trans-Sulfuration Pathway Activity in Astrocytes. Authors - Xinkun Wan View Article
mouse	WB	Neurosci Lett Intracellular metabotropic glutamate receptor 5 in spinal dorsal horn neurons contributes to pain in a mouse model of vincristine-induced neuropathic pain Authors - Xiao Li KD Validated View Article
goat	IHC	Anim Sci J The expression of nutrient chemosensing gate molecules in the ileum and colon is altered for goats fed on a high-grain diet Authors - Changxin Tian View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse ovary using 12686-1-AP

WB analysis of mouse ovary using 12686-1-AP

WB analysis of human brain using 12686-1-AP

WB analysis of mouse testis using 12686-1-AP

IHC staining of mouse brain using 12686-1-AP

IHC staining of mouse brain using 12686-1-AP

IHC staining of human testis using 12686-1-AP

IHC staining of human testis using 12686-1-AP

IHC staining of human brain using 12686-1-AP

IHC staining of human brain using 12686-1-AP

EAAT3 Polyclonal antibody

EAAT3 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Dysregulated Glutamate Transporter SLC1A1 Propels Cystine Uptake via Xc- for Glutathione Synthesis in Lung Cancer.

Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction

Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease.

S-Adenosylmethionine Alleviates Amyloid-β-Induced Neural Injury by Enhancing Trans-Sulfuration Pathway Activity in Astrocytes.

Intracellular metabotropic glutamate receptor 5 in spinal dorsal horn neurons contributes to pain in a mouse model of vincristine-induced neuropathic pain

The expression of nutrient chemosensing gate molecules in the ileum and colon is altered for goats fed on a high-grain diet