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E-cadherin Monoclonal antibody, PBS Only
E-cadherin Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig, rat
Applications
WB, IF, FC, IHC, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
6B11F11
Cat no : 60335-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
Sample-dependent, check data in validation data gallery |
Product Information
60335-1-PBS targets E-cadherin in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.
Tested Reactivity | human, pig, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | E-cadherin fusion protein Ag15085 相同性解析による交差性が予測される生物種 |
Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
Calculated molecular weight | 882 aa, 97 kDa |
Observed molecular weight | 120 kDa |
GenBank accession number | BC141838 |
Gene symbol | CDH1 |
Gene ID (NCBI) | 999 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.