CoraLite® Plus 647-conjugated Drebrin Monoclonal antibody
Drebrin Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1A12H1
Cat no : CL647-67589
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | SH-SY5Y cells |
| Positive FC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL647-67589 targets Drebrin in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Drebrin fusion protein Ag22635 相同性解析による交差性が予測される生物種 |
| Full Name | drebrin 1 |
| Observed molecular weight | 110-120 kDa |
| GenBank accession number | BC007567 |
| Gene symbol | DBN1 |
| Gene ID (NCBI) | 1627 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 Drebrin antibody CL647-67589 | Download protocol |
| FC protocol for CL Plus 647 Drebrin antibody CL647-67589 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
