• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DZIP3 Polyclonal antibody (33563-1-AP)

    WB analysis using 33563-1-AP (same clone as 33563-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33563-1-AP (DZIP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using DZIP3 Polyclonal antibody (33563-1-AP)

    IP experiment of HEK-293T using 33563-1-AP (same clone as 33563-1-PBS)

    IP result of anti-DZIP3 (IP:33563-1-AP, 4ug; Detection:33563-1-AP 1:1000) with HEK-293T cells lysate 2080 ug. This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DZIP3 Polyclonal antibody (33563-1-AP)

    IHC staining of human kidney using 33563-1-AP (same clone as 33563-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 33563-1-AP (DZIP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DZIP3 Polyclonal antibody (33563-1-AP)

    IHC staining of human kidney using 33563-1-AP (same clone as 33563-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 33563-1-AP (DZIP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DZIP3 Polyclonal antibody (33563-1-AP)

    IF Staining of HEK-293 using 33563-1-AP (same clone as 33563-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DZIP3 antibody (33563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DZIP3 Polyclonal antibody (33563-1-AP)

    IF Staining of HeLa using 33563-1-AP (same clone as 33563-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DZIP3 antibody (33563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

DZIP3 Polyclonal antibody, PBS Only

DZIP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 33563-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DAZ-interacting protein 3, E3 ubiquitin-protein ligase DZIP3, EC:2.3.2.27, hRUL138, KIAA0675

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

33563-1-PBS targets DZIP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35815

Product name: Recombinant human DZIP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 631-862 aa of BC063882

Sequence: AEINKDGTSIPSESSTESLKDLQEVKSKQRKKKKTKNKKNKDSKEDQVPYVVEKEEQLRKEQANPHSVSRLIKDDASDVQEDSAMEDKFYSLDELHILDMIEQGSAGKVTTDYGETEKERLARQRQLYKLHYQCEDFKRQLRTVTFRWQENQMQIKKKDKIIASLNQQVAFGINKVSKLQRQIHAKDNEIKNLKEQLSMKRSQWEMEKHNLESTMKTYVSKLNAETSRALTA

相同性解析による交差性が予測される生物種
Full Name DAZ interacting protein 3, zinc finger
Calculated molecular weight 1208 aa, 139 kDa
Observed molecular weight139-150 kDa
GenBank accession numberBC063882
Gene Symbol DZIP3
Gene ID (NCBI) 9666
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86Y13
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DZIP3/hRUL138 is a poorly characterized RNA-binding RING E3-ubiquitin ligase with functions in embryonic development. DZIP3 localizes in both the nucleus and cytoplasm (PMID: 33067265). DZIP3 was mainly reported to be an RNA-binding ligase that causes histone H2A monoubiquitination, which represses transcription. DZIP3 also regulates the differentiation of stem cells (PMID: 32796072).

||
New chat

Able™

正在加载，请稍候...