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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DZIP3 Polyclonal antibody (33563-1-AP)

    WB analysis using 33563-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33563-1-AP (DZIP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using DZIP3 Polyclonal antibody (33563-1-AP)

    IP experiment of HEK-293T using 33563-1-AP

    IP result of anti-DZIP3 (IP:33563-1-AP, 4ug; Detection:33563-1-AP 1:1000) with HEK-293T cells lysate 2080 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DZIP3 Polyclonal antibody (33563-1-AP)

    IHC staining of human kidney using 33563-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 33563-1-AP (DZIP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DZIP3 Polyclonal antibody (33563-1-AP)

    IHC staining of human kidney using 33563-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 33563-1-AP (DZIP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DZIP3 Polyclonal antibody (33563-1-AP)

    IF Staining of HEK-293 using 33563-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DZIP3 antibody (33563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DZIP3 Polyclonal antibody (33563-1-AP)

    IF Staining of HeLa using 33563-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DZIP3 antibody (33563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DZIP3 Polyclonal antibody

DZIP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 33563-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

DAZ-interacting protein 3, E3 ubiquitin-protein ligase DZIP3, EC:2.3.2.27, hRUL138, KIAA0675

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inAC16 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33563-1-AP targets DZIP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35815

Product name: Recombinant human DZIP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 631-862 aa of BC063882

Sequence: AEINKDGTSIPSESSTESLKDLQEVKSKQRKKKKTKNKKNKDSKEDQVPYVVEKEEQLRKEQANPHSVSRLIKDDASDVQEDSAMEDKFYSLDELHILDMIEQGSAGKVTTDYGETEKERLARQRQLYKLHYQCEDFKRQLRTVTFRWQENQMQIKKKDKIIASLNQQVAFGINKVSKLQRQIHAKDNEIKNLKEQLSMKRSQWEMEKHNLESTMKTYVSKLNAETSRALTA

相同性解析による交差性が予測される生物種
Full Name DAZ interacting protein 3, zinc finger
Calculated molecular weight 1208 aa, 139 kDa
Observed molecular weight139-150 kDa
GenBank accession numberBC063882
Gene Symbol DZIP3
Gene ID (NCBI) 9666
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86Y13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DZIP3/hRUL138 is a poorly characterized RNA-binding RING E3-ubiquitin ligase with functions in embryonic development. DZIP3 localizes in both the nucleus and cytoplasm (PMID: 33067265). DZIP3 was mainly reported to be an RNA-binding ligase that causes histone H2A monoubiquitination, which represses transcription. DZIP3 also regulates the differentiation of stem cells (PMID: 32796072).

Protocols

Product Specific Protocols
IF protocol for DZIP3 antibody 33563-1-APDownload protocol
IHC protocol for DZIP3 antibody 33563-1-APDownload protocol
IP protocol for DZIP3 antibody 33563-1-APDownload protocol
WB protocol for DZIP3 antibody 33563-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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