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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of mouse lung using 12890-2-AP (same clone as 12890-2-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12890-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of Jurkat using 12890-2-AP (same clone as 12890-2-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12890-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of U-937 using 12890-2-AP (same clone as 12890-2-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12890-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of mouse testis using 12890-2-AP (same clone as 12890-2-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12890-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of human brain using 12890-2-AP (same clone as 12890-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12890-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DYRK4 Polyclonal antibody (12890-2-AP)

    IHC staining of human lymphoma using 12890-2-AP (same clone as 12890-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12890-2-AP (DYRK4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12890-2-PBS in a different storage buffer formulation.

DYRK4 Polyclonal antibody, PBS Only

DYRK4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12890-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12890-2-PBS targets DYRK4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3515

Product name: Recombinant human DYRK4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 104-400 aa of BC031244

Sequence: YEVLETIGKGSFGQVAKCLDHKNNELVALKIIRNKKRFHQQALMELKILEALRKKDKDNTYNVVHMKDFFYFRNHFCITFELLGINLYELMKNNNFQGFSLSIVRRFTLSVLKCLQMLSVEKIIHCDLKPENIVLYQKGQASVKVIDFGSSCYEHQKVYTYIQSRFYRSPEVILGHPYDVAIDMWSLGCITAELYTGYPLFPGENEVEQLACIMEVLGLPPAGFIQTASRRQTFFDSKGFPKNITNNRGKKRYPDSKDLTMVLKTYDTSFLDFLRRCLVWEPSLRMTPDQALKHAWI

相同性解析による交差性が予測される生物種
Full Name dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
Calculated molecular weight 520 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC031244
Gene Symbol DYRK4
Gene ID (NCBI) 8798
RRIDAB_2094086
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR20
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Dual-specificity tyrosine phosphorylation regulated kinase 4(DYRK4), belongs to a conserved family of serine/threonine protein kinases. DYRK4 is thought to function in the regulation of cell differentiation and proliferation, survival, and in development. DYRK4 has 5 isoforms with molecular weight of 59, 72 and 73 kDa.

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