• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DYRK4 Polyclonal antibody

DYRK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12890-2-AP

Synonyms

DYRK4

Validation Data Gallery

Applications:
  • WB analysis of mouse lung using 12890-2-AP

    WB analysis of mouse lung using 12890-2-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 12890-2-AP

    WB analysis of Jurkat using 12890-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 12890-2-AP

    WB analysis of U-937 using 12890-2-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 12890-2-AP

    WB analysis of mouse testis using 12890-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 12890-2-AP

    WB analysis of human brain using 12890-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 12890-2-AP

    IHC staining of human lymphoma using 12890-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12890-2-AP (DYRK4 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inmouse lung tissue, human brain tissue, Jurkat cells, mouse testis tissue, U-937 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12890-2-AP targets DYRK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYRK4 fusion protein Ag3515 相同性解析による交差性が予測される生物種
Full Name dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
Calculated molecular weight 520 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC031244
Gene symbol DYRK4
Gene ID (NCBI) 8798
RRIDAB_2094086
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dual-specificity tyrosine phosphorylation regulated kinase 4(DYRK4), belongs to a conserved family of serine/threonine protein kinases. DYRK4 is thought to function in the regulation of cell differentiation and proliferation, survival, and in development. DYRK4 has 5 isoforms with molecular weight of 59, 72 and 73 kDa.

Protocols

Product Specific Protocols
WB protocol for DYRK4 antibody 12890-2-APDownload protocol
IHC protocol for DYRK4 antibody 12890-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols