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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of mouse testis using 12240-1-AP (same clone as 12240-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12240-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of mouse skeletal muscle using 12240-1-AP (same clone as 12240-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12240-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of human colon using 12240-1-AP (same clone as 12240-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12240-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of human heart using 12240-1-AP (same clone as 12240-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12240-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of HeLa using 12240-1-AP (same clone as 12240-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12240-1-PBS in a different storage buffer formulation.

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DYRK1B Polyclonal antibody, PBS Only

DYRK1B Polyclonal Antibody for WB, Indirect ELISA
Cat No. 12240-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12240-1-PBS targets DYRK1B in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYRK1B fusion protein Ag2883 相同性解析による交差性が予測される生物種
Full Name dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B
Calculated molecular weight 629 aa, 69 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC018751
Gene Symbol DYRK1B
Gene ID (NCBI) 9149
RRIDAB_2093803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y463
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DYRK1B(dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B) is also named as MIRK. It is a member of the dyrk/minibrain family of serine/threonine kinases that mediate the transition from growth to differentiation in lower eukaryotes and mammals(PMID:15546868). DYRK1B functions as a transcriptional coactivator that is activated by co-expressed MKK3, a MAP kinase that also can activate p38 MAP kinase(PMID:14500717). The DYRK1B splice variants found in each muscle tissue exhibit approximate molecular weights of 70 and 75 kDa(PMID:12902328). It has 3 isoforms produced by alternative splicing of the molecular weight of 65-69 kDa.