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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of mouse testis using 12240-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of mouse skeletal muscle using 12240-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of human colon using 12240-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of human heart using 12240-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DYRK1B Polyclonal antibody (12240-1-AP)

    WB analysis of HeLa using 12240-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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DYRK1B Polyclonal antibody

DYRK1B Polyclonal Antibody for WB, ELISA
Cat No. 12240-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DYRK1B, Minibrain related kinase, MIRK, Mirk protein kinase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, human colon tissue, human heart tissue, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12240-1-AP targets DYRK1B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYRK1B fusion protein Ag2883 相同性解析による交差性が予測される生物種
Full Name dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B
Calculated molecular weight 629 aa, 69 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC018751
Gene Symbol DYRK1B
Gene ID (NCBI) 9149
RRIDAB_2093803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y463
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DYRK1B(dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B) is also named as MIRK. It is a member of the dyrk/minibrain family of serine/threonine kinases that mediate the transition from growth to differentiation in lower eukaryotes and mammals(PMID:15546868). DYRK1B functions as a transcriptional coactivator that is activated by co-expressed MKK3, a MAP kinase that also can activate p38 MAP kinase(PMID:14500717). The DYRK1B splice variants found in each muscle tissue exhibit approximate molecular weights of 70 and 75 kDa(PMID:12902328). It has 3 isoforms produced by alternative splicing of the molecular weight of 65-69 kDa.

Protocols

Product Specific Protocols
WB protocol for DYRK1B antibody 12240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Physiol Cell Physiol

MicroRNA-9 enhances the transactivation of nuclear factor of activated T cells by targeting KPNB1 and DYRK1B.

Authors - Yan Zeng
View Article