Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HEK-293T cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85397-4-RR targets DYNC1H1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | DYNC1H1 fusion protein Ag2999 相同性解析による交差性が予測される生物種 |
| Full Name | dynein, cytoplasmic 1, heavy chain 1 |
| Calculated molecular weight | 4646 aa, 532 kDa |
| Observed molecular weight | 532 kDa |
| GenBank accession number | BC021297 |
| Gene Symbol | DYNC1H1 |
| Gene ID (NCBI) | 1778 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14204 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Dyneins are a group of microtubule-activated ATPases that convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein comprises 2 heavy chain polypeptides and several intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNC1H1 antibody 85397-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |