Validation Data Gallery
![DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.](https://www.ptglab.com/products/pictures/Result-ffak_20211109145130115.png "DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.")
![DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 2x SDS-sample buffer the enriched Flag® fusion protein and Fab-fragment are released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.](https://www.ptglab.com/products/pictures/Result-ffak.png "DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 2x SDS-sample buffer the enriched Flag® fusion protein and Fab-fragment are released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.")
![DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.](https://www.ptglab.com/products/pictures/Result-ffak_20211109145130506.png "DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.")
Product Information
The ChromoTek DYKDDDDK Fab-Trap® Agarose Kit consists of an anti-DYKDDDDK-tag Fab fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap® Agarose Kit is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc
| Description | The DYKDDDDK Fab-Trap Agarose Kit contains DYKDDDDK Fab-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Flag®-tagged proteins and their interacting factors. • Lowest background in class • Less contaminating antibody chains • Recombinantly expressed Fab-fragment derived • Compatible with harsh buffer conditions • Ready-to-use |
| Applications | IP, CoIP, ChIP, RIP, Protein purification |
| Specificity/Target | The DYKDDDDK Fab-Trap® Agarose specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). It binds to same epitope as Sigma's Anti-FLAG® Antibody and is compatible with N-, C-terminal or internal tagging. |
| Binding capacity | 12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | 3xDYKDDDDK-peptide SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 0.5 % Nonidet P40 Substitute, 5 % Tween™ 20, 2 M NaCl, 10 mM TCEP, 10 mM DTT, 10 mM ß-mercaptoethanol, 2 M Urea |
| Type | Fab-fragment |
| Class | Recombinant |
| Host | Mouse |
| RRID | AB_2894836 |
| Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| DYKDDDDK Fab-Trap™ Agarose | 20 reactions (500 µL slurry) |
Documentation
| SDS |
|---|
| ffak_SDS_DYKDDDDK Fab-Trap® Agarose Kit (EN) |
| Datasheet |
|---|
| Protocol DYKDDDDK Fab-Trap® Agarose Kit (PDF) |
Publications
| Application | Title |
|---|---|
Cell Chem Biol An engineered cereblon optimized for high throughput screening and molecular glue discovery | |
Nat Commun A spatio-temporally constrained gene regulatory network directed by PBX1/2 acquires limb patterning specificity via HAND2 | |
Sci Adv Nuclear pyruvate dehydrogenase complex regulates histone acetylation and transcriptional regulation in the ethylene response |