Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13444-1-AP (same clone as 13444-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 13444-1-AP (same clone as 13444-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IP experiment of Raji using 13444-1-AP (same clone as 13444-1-PBS)
IP result of anti-DVL3 (IP:13444-1-AP, 4ug; Detection:13444-1-AP 1:500) with Raji cells lysate 1440ug. This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 13444-1-AP (same clone as 13444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 13444-1-AP (same clone as 13444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 13444-1-AP (same clone as 13444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 13444-1-AP (same clone as 13444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 13444-1-AP (same clone as 13444-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 13444-1-AP (same clone as 13444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 13444-1-AP (same clone as 13444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 13444-1-AP (same clone as 13444-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 13444-1-AP (same clone as 13444-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DVL3 antibody (13444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 13444-1-AP (same clone as 13444-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13444-1-AP (DVL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

DVL3 Polyclonal antibody, PBS Only

DVL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13444-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13444-1-PBS targets DVL3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4272 Product name: Recombinant human DVL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-319 aa of BC032459 Sequence: MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAEGSHPDPAPFCADNPSELPPPMERTGGIGDSRPPSFHPHAGGGSQENLDNDTETDSLVSAQRERPRRRDGPEHATRLNGTAKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVL 相同性解析による交差性が予測される生物種
Full Name	dishevelled, dsh homolog 3 (Drosophila)
Calculated molecular weight	716 aa, 78 kDa
Observed molecular weight	78 kDa
GenBank accession number	BC032459
Gene Symbol	DVL3
Gene ID (NCBI)	1857
RRID	AB_2093451
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92997
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13444-1-AP (same clone as 13444-1-PBS)

WB analysis of MCF-7 using 13444-1-AP (same clone as 13444-1-PBS)

IP experiment of Raji using 13444-1-AP (same clone as 13444-1-PBS)

IHC staining of human colon cancer using 13444-1-AP (same clone as 13444-1-PBS)

IHC staining of human colon cancer using 13444-1-AP (same clone as 13444-1-PBS)

IHC staining of human cervical cancer using 13444-1-AP (same clone as 13444-1-PBS)

IHC staining of human cervical cancer using 13444-1-AP (same clone as 13444-1-PBS)

IHC staining of mouse colon using 13444-1-AP (same clone as 13444-1-PBS)

IHC staining of human prostate cancer using 13444-1-AP (same clone as 13444-1-PBS)