DVL3 Polyclonal antibody
DVL3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13444-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | A549 cells, MCF-7 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human colon cancer tissue, human prostate cancer tissue, mouse colon tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy DVL3 Ready-To-Use IHC Kit. DVL3 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
13444-1-AP targets DVL3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DVL3 fusion protein Ag4272 相同性解析による交差性が予測される生物種 |
| Full Name | dishevelled, dsh homolog 3 (Drosophila) |
| Calculated molecular weight | 716 aa, 78 kDa |
| Observed molecular weight | 78 kDa |
| GenBank accession number | BC032459 |
| Gene symbol | DVL3 |
| Gene ID (NCBI) | 1857 |
| RRID | AB_2093451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DVL3 antibody 13444-1-AP | Download protocol |
| IHC protocol for DVL3 antibody 13444-1-AP | Download protocol |
| IF protocol for DVL3 antibody 13444-1-AP | Download protocol |
| IP protocol for DVL3 antibody 13444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled. | ||
J Cell Biol Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling. | ||
Oncogene The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer. | ||
Cancer Lett SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network. | ||
Oncotarget Dalbinol, a rotenoid from Amorpha fruticosa L., exerts anti-proliferative activity by facilitating β-catenin degradation in hepatocellular carcinoma cells. | ||
Biol Proced Online Circadian gene CSNK1D promoted the progression of hepatocellular carcinoma by activating Wnt/β-catenin pathway via stabilizing Dishevelled Segment Polarity Protein 3 |
