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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DVL1 Polyclonal antibody (27384-1-AP)

    WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DVL1 Polyclonal antibody (27384-1-AP)

    WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DVL1 Polyclonal antibody (27384-1-AP)

    WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DVL1 Polyclonal antibody (27384-1-AP)

    WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using DVL1 Polyclonal antibody (27384-1-AP)

    WB analysis of NIH/3T3 using 27384-1-AP (same clone as 27384-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using DVL1 Polyclonal antibody (27384-1-AP)

    WB analysis of mouse cerebellum using 27384-1-AP (same clone as 27384-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using DVL1 Polyclonal antibody (27384-1-AP)

    IP experiment of HEK-293 using 27384-1-AP (same clone as 27384-1-PBS)

    IP result of anti-DVL1 (IP:27384-1-AP, 4ug; Detection:27384-1-AP 1:3000) with HEK-293 cells lysate 1560 ug. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DVL1 Polyclonal antibody (27384-1-AP)

    IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DVL1 Polyclonal antibody (27384-1-AP)

    IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DVL1 Polyclonal antibody (27384-1-AP)

    IHC staining of mouse colon using 27384-1-AP (same clone as 27384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DVL1 Polyclonal antibody (27384-1-AP)

    IHC staining of mouse colon using 27384-1-AP (same clone as 27384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DVL1 Polyclonal antibody (27384-1-AP)

    IHC staining of human prostate cancer using 27384-1-AP (same clone as 27384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DVL1 Polyclonal antibody (27384-1-AP)

    IHC staining of human prostate cancer using 27384-1-AP (same clone as 27384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DVL1 Polyclonal antibody (27384-1-AP)

    IF Staining of MCF-7 using 27384-1-AP (same clone as 27384-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DVL1 antibody (27384-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DVL1 Polyclonal antibody, PBS Only

DVL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 27384-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Dishevelled 1, Dishevelled-1, DSH homolog 1, DVL, Segment polarity protein dishevelled homolog DVL-1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27384-1-PBS targets DVL1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26055

Product name: Recombinant human DVL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 283-416 aa of BC050454

Sequence: LGQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSGSESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRELA

相同性解析による交差性が予測される生物種
Full Name dishevelled, dsh homolog 1 (Drosophila)
Calculated molecular weight 75 kDa
Observed molecular weight73-75 kDa
GenBank accession numberBC050454
Gene Symbol DVL1
Gene ID (NCBI) 1855
RRIDAB_2880859
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14640
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.

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