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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP6 Polyclonal antibody (26071-1-AP)

    WB analysis using 26071-1-AP (same clone as 26071-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26071-1-AP (DUSP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-12 cells using DUSP6 Polyclonal antibody (26071-1-AP)

    IP experiment of PC-12 using 26071-1-AP (same clone as 26071-1-PBS)

    IP result of anti-DUSP6 (IP:26071-1-AP, 4ug; Detection:26071-1-AP 1:1000) with PC-12 cells lysate 1040 ug. This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using DUSP6 Polyclonal antibody (26071-1-AP)

    IHC staining of mouse pancreas using 26071-1-AP (same clone as 26071-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 26071-1-AP (DUSP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DUSP6 Polyclonal antibody (26071-1-AP)

    IF Staining of HeLa using 26071-1-AP (same clone as 26071-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DUSP6 antibody (26071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using DUSP6 Polyclonal antibody (26071-1-AP)

    IF Staining of PC-12 using 26071-1-AP (same clone as 26071-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using DUSP6 antibody (26071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DUSP6 Polyclonal antibody (26071-1-AP)

    FC experiment of HeLa using 26071-1-AP (same clone as 26071-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUSP6 Polyclonal antibody (26071-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

DUSP6 Polyclonal antibody, PBS Only

DUSP6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 26071-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

MKP3, MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26071-1-PBS targets DUSP6 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23474

Product name: Recombinant human DUSP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-381 aa of BC003562

Sequence: CDNRVPAQQLYFTTPSNQNVYQVDSLQST

相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 6
Calculated molecular weight42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC003562
Gene Symbol DUSP6
Gene ID (NCBI) 1848
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16828
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).

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