Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, PC-12 cells, mouse heart tissue |
| Positive IP detected in | PC-12 cells |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, PC-12 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
26071-1-AP targets DUSP6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23474 Product name: Recombinant human DUSP6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 353-381 aa of BC003562 Sequence: CDNRVPAQQLYFTTPSNQNVYQVDSLQST 相同性解析による交差性が予測される生物種 |
| Full Name | dual specificity phosphatase 6 |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC003562 |
| Gene Symbol | DUSP6 |
| Gene ID (NCBI) | 1848 |
| RRID | AB_3669521 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16828 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DUSP6 antibody 26071-1-AP | Download protocol |
| IHC protocol for DUSP6 antibody 26071-1-AP | Download protocol |
| IP protocol for DUSP6 antibody 26071-1-AP | Download protocol |
| WB protocol for DUSP6 antibody 26071-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
