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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated DSP Monoclonal antibody

DSP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B10D4

Cat no : CL488-68364

Synonyms

desmoplakin, DP, DPI, DPII, DSP

Validation Data Gallery

Applications:
  • IF Staining of A431 using CL488-68364

    IF Staining of A431 using CL488-68364

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 DSP antibody (CL488-68364, Clone: 1B10D4 ) at dilution of 1:200.

Tested Applications

Positive IF detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68364-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-68364 targets DSP in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DSP fusion protein Ag30499 相同性解析による交差性が予測される生物種
Full Name desmoplakin
Calculated molecular weight 2871 aa, 332 kDa
Observed molecular weight 240-330 kDa
GenBank accession numberBC140802
Gene symbol DSP
Gene ID (NCBI) 1832
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DSP antibody CL488-68364Download protocol
Standard Protocols
Click here to view our Standard Protocols