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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DSP Monoclonal antibody

DSP Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10D4

Cat no : 68364-1-Ig

Synonyms

desmoplakin, DP, DPI, DPII, DSP

Validation Data Gallery

Applications:
  • WB analysis using 68364-1-Ig

    WB analysis using 68364-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68364-1-Ig (DSP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 68364-1-Ig

    IHC staining of mouse heart using 68364-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 68364-1-Ig

    IHC staining of mouse heart using 68364-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 68364-1-Ig

    IF Staining of A431 using 68364-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSP antibody (68364-1-Ig, Clone: 1B10D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, A431 cells, MCF-7 cells, HEK-293 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

68364-1-Ig targets DSP in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DSP fusion protein Ag30499 相同性解析による交差性が予測される生物種
Full Name desmoplakin
Calculated molecular weight 2871 aa, 332 kDa
Observed molecular weight 240-330 kDa
GenBank accession numberBC140802
Gene symbol DSP
Gene ID (NCBI) 1832
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

Protocols

Product Specific Protocols
WB protocol for DSP antibody 68364-1-IgDownload protocol
IHC protocol for DSP antibody 68364-1-IgDownload protocol
IF protocol for DSP antibody 68364-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols