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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DSG3 Polyclonal antibody (29942-1-AP)

    WB analysis using 29942-1-AP (same clone as 29942-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29942-1-AP (DSG3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using DSG3 Polyclonal antibody (29942-1-AP)

    IHC staining of mouse tongue using 29942-1-AP (same clone as 29942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using DSG3 Polyclonal antibody (29942-1-AP)

    IHC staining of mouse tongue using 29942-1-AP (same clone as 29942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung squamous cell carcinoma tissue using DSG3 Polyclonal antibody (29942-1-AP)

    IHC staining of human lung squamous cell carcinoma using 29942-1-AP (same clone as 29942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using DSG3 Polyclonal antibody (29942-1-AP)

    IF Staining of HaCaT using 29942-1-AP (same clone as 29942-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using DSG3 antibody (29942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using DSG3 Polyclonal antibody (29942-1-AP)

    IF Staining of HaCaT using 29942-1-AP (same clone as 29942-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using DSG3 antibody (29942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

DSG3 Polyclonal antibody, PBS Only

DSG3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29942-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PVA, DSG 3, Desmoglein-3, Desmoglein 3, CDHF6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29942-1-PBS targets DSG3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30880

Product name: Recombinant human DSG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 760-880 aa of NM_001944

Sequence: SGQSGTMRTRHSTGGTNKDYADGAISMNFLDSYFSQKAFACAEEDDGQEANDCLLIYDNEGADATGSPVGSVGCCSFIADDLDDSFLDSLGPKFKKLAEISLGVDGEGKEVQPPSKDSGYG

相同性解析による交差性が予測される生物種
Full Name desmoglein 3 (pemphigus vulgaris antigen)
Calculated molecular weight 108 kDa
Observed molecular weight130 kDa
GenBank accession numberNM_001944
Gene Symbol DSG3
Gene ID (NCBI) 1830
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP32926
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DSG3, Desmoglein-3, is the component of intercellular desmosome junctions. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSG3 is expressed in the basal lower layers of in the skin epidermis (PMID: 30528827). It acts as a prognostic marker of Esophageal Squamous cell carcinoma (PMID:24568510).

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