×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DSG3 Polyclonal antibody (29942-1-AP)

    WB analysis using 29942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29942-1-AP (DSG3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using DSG3 Polyclonal antibody (29942-1-AP)

    IHC staining of mouse tongue using 29942-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using DSG3 Polyclonal antibody (29942-1-AP)

    IHC staining of mouse tongue using 29942-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung squamous cell carcinoma tissue using DSG3 Polyclonal antibody (29942-1-AP)

    IHC staining of human lung squamous cell carcinoma using 29942-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using DSG3 Polyclonal antibody (29942-1-AP)

    IF Staining of HaCaT using 29942-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using DSG3 antibody (29942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using DSG3 Polyclonal antibody (29942-1-AP)

    IF Staining of HaCaT using 29942-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using DSG3 antibody (29942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

DSG3 Polyclonal antibody

DSG3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29942-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PVA, DSG 3, Desmoglein-3, Desmoglein 3, CDHF6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HaCaT cells
Positive IHC detected inmouse tongue tissue, human lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29942-1-AP targets DSG3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30880

Product name: Recombinant human DSG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 760-880 aa of NM_001944

Sequence: SGQSGTMRTRHSTGGTNKDYADGAISMNFLDSYFSQKAFACAEEDDGQEANDCLLIYDNEGADATGSPVGSVGCCSFIADDLDDSFLDSLGPKFKKLAEISLGVDGEGKEVQPPSKDSGYG

相同性解析による交差性が予測される生物種
Full Name desmoglein 3 (pemphigus vulgaris antigen)
Calculated molecular weight 108 kDa
Observed molecular weight130 kDa
GenBank accession numberNM_001944
Gene Symbol DSG3
Gene ID (NCBI) 1830
RRIDAB_3086196
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP32926
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DSG3, Desmoglein-3, is the component of intercellular desmosome junctions. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSG3 is expressed in the basal lower layers of in the skin epidermis (PMID: 30528827). It acts as a prognostic marker of Esophageal Squamous cell carcinoma (PMID:24568510).

Protocols

Product Specific Protocols
IF protocol for DSG3 antibody 29942-1-APDownload protocol
IHC protocol for DSG3 antibody 29942-1-APDownload protocol
WB protocol for DSG3 antibody 29942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Signal

ZCRB1 promotes proliferation and metastasis of lung squamous cell carcinoma by stabilizing DSG3 mRNA.

Authors - Hang Hou
View Article
humanWB

Discov Oncol

Identification of KRT16 and ANXA10 as cell cycle regulation genes for lung adenocarcinoma based on self-transcriptome sequencing of surgical samples and TCGA public data mining

Authors - Wen-Jian Liu
View Article
||
New chat

Able™

正在加载，请稍候...