Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HaCaT cells |
| Positive IHC detected in | mouse tongue tissue, human lung squamous cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HaCaT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
29942-1-AP targets DSG3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30880 Product name: Recombinant human DSG3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 760-880 aa of NM_001944 Sequence: SGQSGTMRTRHSTGGTNKDYADGAISMNFLDSYFSQKAFACAEEDDGQEANDCLLIYDNEGADATGSPVGSVGCCSFIADDLDDSFLDSLGPKFKKLAEISLGVDGEGKEVQPPSKDSGYG 相同性解析による交差性が予測される生物種 |
| Full Name | desmoglein 3 (pemphigus vulgaris antigen) |
| Calculated molecular weight | 108 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | NM_001944 |
| Gene Symbol | DSG3 |
| Gene ID (NCBI) | 1830 |
| RRID | AB_3086196 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32926 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DSG3, Desmoglein-3, is the component of intercellular desmosome junctions. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSG3 is expressed in the basal lower layers of in the skin epidermis (PMID: 30528827). It acts as a prognostic marker of Esophageal Squamous cell carcinoma (PMID:24568510).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DSG3 antibody 29942-1-AP | Download protocol |
| IHC protocol for DSG3 antibody 29942-1-AP | Download protocol |
| WB protocol for DSG3 antibody 29942-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |