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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated DSE Monoclonal antib (CL488-60041)

    IF Staining of HeLa using CL488-60041

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DSE antibody (CL488-60041, Clone: 2C9F7 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated DSE Monoclonal antib (CL488-60041)

    FC experiment of HeLa using CL488-60041

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DSE Monoclonal antibody (CL488-60041, Clone:2C9F7)(red), or 0.4 ug Isotype Control (CL488-66360-1) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated DSE Monoclonal antibody

DSE Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-60041
Clone No.2C9F7

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

EC:5.1.3.19, DSEPI, DS epimerase, Dermatan-sulfate epimerase, Chondroitin-glucuronate 5-epimerase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60041 targets DSE in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen DSE fusion protein Ag0695 相同性解析による交差性が予測される生物種
Full Name dermatan sulfate epimerase
Calculated molecular weight 958 aa, 110 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC039245
Gene Symbol DSE
Gene ID (NCBI) 29940
RRIDAB_3672860
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodEuglobulin precipitation
UNIPROT IDQ9UL01
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DSE antibody CL488-60041Download protocol
FC protocol for CL Plus 488 DSE antibody CL488-60041Download protocol
Standard Protocols
Click here to view our Standard Protocols