Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
33922-1-PBS targets DPH5 in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38953 Product name: Recombinant human DPH5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-285 aa of NM_001077394.1 Sequence: WRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL 相同性解析による交差性が予測される生物種 |
| Full Name | DPH5 homolog (S. cerevisiae) |
| Calculated molecular weight | 32kDa，285aa |
| Observed molecular weight | 26-36 kDa |
| GenBank accession number | NM_001077394.1 |
| Gene Symbol | DPH5 |
| Gene ID (NCBI) | 51611 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H2P9 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
The research background of DPH5 mainly revolves around its key role in diphthamide amide biosynthesis pathway. Diphtheria amide is a highly conserved post-translational modification of eukaryotic translation elongation factor eEF2, which is very important for maintaining translation accuracy and protein synthesis efficiency. Due to modification, the molecular weight is 26-36 kDa. There are also some isoform and ubiquitination modifications.