Validation Data Gallery
Tested Applications
| Positive WB detected in | A-204 cells, HL-60 cells, Raji cells, Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33922-1-AP targets DPH5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38953 Product name: Recombinant human DPH5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-285 aa of NM_001077394.1 Sequence: WRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL 相同性解析による交差性が予測される生物種 |
| Full Name | DPH5 homolog (S. cerevisiae) |
| Calculated molecular weight | 32kDa，285aa |
| Observed molecular weight | 26-36 kDa |
| GenBank accession number | NM_001077394.1 |
| Gene Symbol | DPH5 |
| Gene ID (NCBI) | 51611 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H2P9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The research background of DPH5 mainly revolves around its key role in diphthamide amide biosynthesis pathway. Diphtheria amide is a highly conserved post-translational modification of eukaryotic translation elongation factor eEF2, which is very important for maintaining translation accuracy and protein synthesis efficiency. Due to modification, the molecular weight is 26-36 kDa. There are also some isoform and ubiquitination modifications.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DPH5 antibody 33922-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |