Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of A549 using 18987-1-AP (same clone as 18987-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
WB analysis using 18987-1-AP (same clone as 18987-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 18987-1-AP (same clone as 18987-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 18987-1-AP (same clone as 18987-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 18987-1-AP (same clone as 18987-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 18987-1-AP (same clone as 18987-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 18987-1-AP (same clone as 18987-1-PBS)
IP result of anti-DOCK9 (IP:18987-1-AP, 4ug; Detection:18987-1-AP 1:800) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18987-1-AP (same clone as 18987-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18987-1-AP (same clone as 18987-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 18987-1-AP (same clone as 18987-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 18987-1-AP (same clone as 18987-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 18987-1-AP (same clone as 18987-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK9 antibody (18987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

DOCK9 Polyclonal antibody, PBS Only

DOCK9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 18987-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Zizimin-1, ZIZ1, dedicator of cytokinesis 9, Cdc42 guanine nucleotide exchange factor zizimin-1

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18987-1-PBS targets DOCK9 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	dedicator of cytokinesis 9
Calculated molecular weight	239 kDa
Observed molecular weight	200-236 kDa
GenBank accession number	NM_015296
Gene Symbol	DOCK9
Gene ID (NCBI)	23348
RRID	AB_10596777
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZ29
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 18987-1-AP (same clone as 18987-1-PBS)

WB analysis using 18987-1-AP (same clone as 18987-1-PBS)

WB analysis of human heart using 18987-1-AP (same clone as 18987-1-PBS)

WB analysis of mouse brain using 18987-1-AP (same clone as 18987-1-PBS)

WB analysis of human placenta using 18987-1-AP (same clone as 18987-1-PBS)

WB analysis of human brain using 18987-1-AP (same clone as 18987-1-PBS)

IP experiment of mouse brain using 18987-1-AP (same clone as 18987-1-PBS)

IHC staining of human kidney using 18987-1-AP (same clone as 18987-1-PBS)

IHC staining of human kidney using 18987-1-AP (same clone as 18987-1-PBS)

IHC staining of human heart using 18987-1-AP (same clone as 18987-1-PBS)